= Satellite meeting for Use-cases development and documentation =

== Topics ==


== Participants ==

 * Toshiaki Katayama
 * Young Joo Kim
 * Keun-Joon Park
 * Yunsun Nam
 * Arek Kasprzyk 
 * Syed Haider
 * Shuichi Kawashima
 * Takeshi Kawashima
 * Raoul JP Bonnal
 * Tatsuya Nishizawa
 * Oswaldo Trelles 
 * José M. Fernández
 * Paul Gordon 
 * Vachiranee Limviphuvadh
 * Tobias Gattermayer
 * Riu Yamashita 
 * Fumikazu Konishi 

== Targets ==

 

== Date ==

 * 2009/3/19 15:00-18:00
 * anytime


== Room ==

 * 3F 

== Notes ==

'''Data for testing'''

1) SNPs data contain 262,338 SNPs (Stroke patient vs. normal) from Affymetrix GeneChip (provided by Prof.Kim)[[BR]]
2) Amino acid sequences of 53 genes (multifasta format) which are located at disease map locus of one form of epilepsy (provided by Vachiranee)[[BR]]
3) Nucleotide sequences (multifasta format) (provided by Riu)[[BR]]
4) Genome data (provided by Takeshi)[[BR]]


'''Softwares''' 

1) BioMart[[BR]]
2) Galaxy[[BR]]
3) jORCA[[BR]]
4) ANNOTATOR[[BR]]


== Results ==
  
'''*SNPs data'''[[BR]]

ANNOTATOR currently cannot analyse SNPs data [[BR]]
Galaxy (http://galaxyproject.org) and RGenetics (http://rgenetics.org/)
        quality control, ancestry, case-control analysis, tdt, oter statistical tests

'''*Multifasta format (amino acid sequences)'''[[BR]]

'''*Multifasta format (nucleotide sequences)''' [[BR]]

'''*Genome data'''[[BR]]

Question
- Takeshi asked about how to annoate in-house data with comparing with public genome data using BioMart.
- For example, how to annotate Halocynthia roretzi or Molgula tectiformis ESTs comparing with Ciona intestinalis and Ciona savigni using BioMart.
Answer
- Convert Halocynthia data into BioMart format. Install BioMart server locally. Then merge public DB and local data together.
- BioMart format is simple.


Takeshi present an example
- minor animal (ex, H.roretzi, closest animal of Ciona)
- how can analyze Halocynthia roretzi and M.tectiformis which are rare annotation.
- type of data is ESTs, assembled EST cluster,,,etc,,
- potential flow: BioMart -> ANNOTATOR -> TogoDB

DL ESTs of Halocynthia roretzi from NCBI/Taxonomy

TogoDB by Toshiaki
- upload table format  (table services for uploading data will be able to access using WS)


jORCA by Oswaldo

-mapped WABI services
- jORCA: WABI WSDL application can run in local machine after installation
- jORCA can tell which kind of analysis can do with our multifasta format file or anykind of format. For example, using Magallanes: INB*,
if put "FASTA" in Find box, the result come up with 23 tools that can use for analysis. For analysis use myexperiment.org
*Magallanes: INB (Services discovering that discover what kind of analysis can provide for your data format)


ANNOTATOR

- how different between ANNOTATOR and InterProscan
- how can I know the version of each DB use in Prim-seq-algorithm
- it would be nice if user can save result in xml format



== TODOs ==


== Other requests from use case to developper of any WS ==

- (TO: IntAct, Cytoscape) It would be nice if user can retrive PPIs which are expressed in any tissues by using option function. (From: Vachiranee)