Satellite meeting for Transcription regulation

Topics

Biologists need to get more detailed information about what is happening a gene level when they look at microarray data:

• Enrichment analysis of TFBS
• Transcription factor binding site SNPs
• Histone binding
• Network analysis (which genes are interesting??)
• Expression levels
• siRNA, miRNA that control function
• Methylation
• Copy number variation
• Protein structure of TFs

Attendees

• Riu Yamashita
• Young Joo Kim
• Alberto Labarga
• Yulia Kovarskaya

Objective

Make a unified solution tool that biologists want in their functional, molecular studies. -Identify feasible techniques and economic solutions for them.

The questions that should be addressed.

1. Transcription binding sites, modulations

2. Histon binding sites

3. Histon modification

4. SNPs, SNP chips

5. Expression (mRNA or cDNA chips)

6. miRNA or RNAi

7. methylation (epigenetics)

8. CNV (copy number variation)

9. Protein structure

10. Protein chips

11. Tissue arrays

12. Cell-level functional studies

13. Pathway analysis

14. Next generation sequencing

15. OMIM or disease DB

16. Comparative genomics

17. Technologies unknown yet

How: Define each question in terms of biologists’ interests. Describe the limits of each question. Find the best or second best tools for each question. How to combine the questions for the sake of biologists (input and output data)

Proposed Solution

Use DAS'''

• You can find an  introduction for the DAS system here
• The DAS workshop 2009 is just over, and there was some cool stuff there we could use  DAS workshop2009
• What we want to achieve is quite similar to this:  http://beta.gepas.bioinfo.cipf.es/pupasuite/www/index.jsp --Surely, it is really what I want. But it is to slow, so it is hard to analyze many times (Riu)

Results

• Implemented a DAS layer for  FESD II and the TFBS prediction systems
• Implemented a web application that computes the enrichment of the TFBS for a list of genes or proteins, and present the results using  Ajax DAS viewer

Results

Future Plans

• Make some web server to evaluate the specific gene groups.