Changes between Version 16 and Version 17 of DDBJ-KEGG-PDBj
- Timestamp:
- 2009/03/18 11:24:34 (16 years ago)
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DDBJ-KEGG-PDBj
v16 v17 20 20 21 21 === Part I === 22 Using KEGG API: http://www.genome.jp/kegg/soap/ 23 22 24 1. Pick a pathway of a specific organism, say Glycolysis / Gluconeogenesis pathway of Homo sapiens (human). 23 25 1. Get all the amino acid sequences of that pathway component (c''1'', c''2'', ..., c''m''). 24 26 25 27 === Part II === 28 Using WABI (DDBJ): http://xml.nig.ac.jp/index.html 29 26 30 1. Run blast for each sequence against !UniProt(!SwissProt / TrEMBL). 27 31 1. Group the blast results according to biological species (s''1'', s''2'', ..., s''n''). … … 29 33 * At this step, you have (at most) an ''m'' compoents X ''n'' species array of protein sequences: '''Homologous Compoenents Matrix''' (HCM). 30 34 === Part III === 35 Using Sequence Navigator API (PDBj): http://pdbjs3.protein.osaka-u.ac.jp/seqnavix/soap/index.html 36 31 37 1. Pick one column (species) of the HCM (s''j'') For each component of that column, run blast against PDB sequences. 32 38 * You will have a list of PDBID and Chain ID as a result of this blast search. … … 97 103 98 104 105 106