Version 2 (modified by akinjo, 15 years ago)

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Creating an example of workflows using DDBJ-KEGG-PDBj

Member: Shigemoto (DDBJ) and Kinjo (PDBj) with some help from the KEGG people.

What we want to do

Finding pathway components (enzymes) that are expected to form physical contacts as judged from homologous protein structures.

The procedure

1. Pick a pathway of a specific organism, say Glycolysis / Gluconeogenesis pathway of Homo sapiens (human). 1. Get all the amino acid sequences of that pathway component. 1. Run blast for each sequence against UniProt?(SwissProt? / TrEMBL). 1. Group the blast results according to biological species (s1, s2, ..., sn).

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